Western Blot Topics

• bovine serum albumin
• bovine serum albumin bsa
• target proteins
• target protein
• cellular studies
• dilute solution
• dry milk
• detergent
• antibody
• bacteria
• tween 20
• virus

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Western Blot, Western Blot

However, it should be noted that bacteria, virus or environmental samples can be the source of protein and thus Western blotting is not restricted to cellular studies only.

Blocking of non-specific binding is achieved by placing the membrane in a dilute solution of protein - typically Bovine serum albumin (BSA) or non-fat dry milk (both are inexpensive), with a minute percentage of detergent such as Tween 20. The protein in the dilute solution attaches to the membrane in all places where the target proteins have not attached. Thus, when the antibody is added, there is no room on the membrane for it to attach other than on the binding sites of the specific target protein. This reduces "noise" in the final product of the Western blot, leading to clearer results, and eliminates false positives.

Source: Wikipedia > Western Blot