Okazaki Fragment Topics

• okazaki fragments
• nucleotides
• radioactivity
• centrifuge
• chases
• e coli
• strands
• replication
• dna
• lagging strand
• synthesis
• pulse

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Okazaki Fragment, Okazaki Fragment

The lengths of Okazaki fragments are between 1,000 to 2,000 nucleotides long in E. coli and are generally between 100 to 200 nucleotides long in eukaryotes.

Regarding the lagging strand, the result of this strand's discontinuous replication is the production of a series of short sections of DNA called Okazaki fragments.

After the pulse Okazaki chased with "cold" un-labeled nucleotides for varying amounts of time and quickly isolated the DNA. Then the DNA was centrifuged and analyzed for radioactivity. What Okazaki found was that with short chases of about 7 to 15 seconds most of the radioactivity was found in the small fragments higher in the tube after centrifuge. However with longer chases more radioactivity was found in the lower, larger strands. This confirmed that during synthesis first small fragments are formed on the lagging strand, then later these fragments are combined and incorporated into much larger strands. The small fragments found on the lagging strand are called Okazaki fragments.

Source: Wikipedia > Okazaki Fragment